Enzymes Lab

Lab: Enzymes Experiment 1: Effect of pH on Enzyme military action Date Objective: To study the action of pH on enzymatic employment. Material Required: 1. Catalase enzyme 2. piss system2 3. Scaled Beaker 4. Dropper 5. Acidic antecedent 6. Basic solution theory Does change in pH affects an enzymatic occupation? scrutiny of Hypothesis Enzyme may stick egress change by various factors. pH is also one out of these? It could be analyzed via introduction of change in pH specialize via addition of contrary hot and base in reaction mixture. For instance, in the reaction mixture of catalase with its substrate much(prenominal) as; H2O2. The reaction of Catalase enzyme on its substrate might be represented as follows: H2O2=H2O+1/2O2 If pH go away affect its activity thusly due to change in O2 production the direct of spume would also change. information collection Data might be collected via measurement of the aim of f oam in the scaled beaker. turn off 1: Effect of pH on Enzyme activity Tube| pH| Level of foam (in mm.)| | | 1| 2| 4| | | 2| 14| 1| | | 3| 8| 102| | | Data summary Data analysis may be done via conclusion a relation between changed pH control and level of O2 produced in this way. Result According to the data, there is reduction in activity of enzyme in acidic (pH = 2) and highly basic condition (pH = 14) due to slight foam (means less O2) production. But, at or so basic condition (pH=8) the activity of enzyme is highest. Graphical Representation Enzyme activity (y axis) depends on entertain of pH (x-axis) hence, there might be detect a flower in graph where the value of enzymatic activity is maximum. O 8 Discussion The electrical shock of pH on enzymatic activity might be due to change in the ionisation of amino acid residues and disruption in the non-covalent interacti! ons. Conclusion Hence; it could be observed that our hypothesis that pH might also...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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